Author(s):

Deeptirekha Behera, Pallavi Mangaraj, Alaka Swain and Mirza Jaynul Baig

Abstract: Continuous effort to improve in vitro culture and genetic transformation system are needed to increase the rice yield. In this study, three widely cultivated rice varieties (Naveen, IR64 and Swarna) were tested on six different media for callus induction. Similarly, three different regeneration media were examined for their efficiency in shoot induction. The MS medium supplemented with 3mgL^-1 BAP, 1.0mgL^-1 kinetin and 0.5 mgL^-1 NAA showed better regeneration efficiency in IR64 and Swarna. In case of Naveen variety, maximum green shoot regeneration was achieved on MS medium supplemented with 2.0 mgL^-1 Kinetin and 0.5 mgL^-1 NAA. All the regenerated plantlets developed good root system in MS medium with 1.0mgL^-1 NAA. Concentration of acetosyringone (AS) were optimized for Agrobacterium mediated transformation. 100μM AS in co-cultivation media was found to be the optimum concentration for Naveen, whereas 150μM AS was recorded as suitable for IR64 and Swarna. The results show that the modified media significantly improved the callus induction, regeneration and transformation efficiency of all three cultivars tested.

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