Author(s):

Krushnanjali Arvind Pagare, Dr. Annasaheb Navale, Dr. Chintamani Deokar and Arjun Awale

Abstract: The present study was focused on isolation, selection and characterization salt tolerant Rhizobium from root nodules of soybean. Out of fourty samples total thirty three rhizobial isolates were obtained and maintained on Yeast Extract Manitol Agar (YEMA) medium. For assessment of salt tolerancy the medium was supplemented with different NaCl concentrations, MgCl₂ concentration and different pH level. All the isolates showed promising salt tolerance. Out of thirty three isolates twenty isolates were extremely salt tolerant (more than 5.4 % salt tolerance limit). Four isolate were tolerant to NaCl concentration (2.10- 3.6 % salt). Nine isolates were moderately tolerant to NaCl concentration (0.09-1.50 % salt). Classification of salt tolerant Rhizobium was done on the basis of salt tolerance limit. Out of thirty three isolates, twenty isolates showed growth at MgCl₂ concentration of 0.75 %, four isolates showed growth at 0.50 % MgCl₂ concentration and nine isolates showed growth at 0.40 % MgCl₂ concentration. Among thirty three isolates, twenty isolates showed growth at 8.50, four isolates showed growth at pH 8.00 and nine isolates showed growth at pH 7.50. Out of thirty three isolates twenty isolates were classified under extremely salt tolerant were characterized for morphological, cultural, physiological and biochemical characterization. The work was carried out for the isolation, selection and characterization of salt tolerant strains of Rhizobium which would be highly important inoculum to improve the growth and development of the leguminous plant under saline environment. There is currently need to develop highly salt-tolerant Rhizobium strains.

Pages: 1976-1982  |  947 Views  234 Downloads

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