Author(s):

Babu AG, Prabhuling G, Rashmi S Karani, SATISH D, Rohini K Patil, Mulla SR, Raghavendra G, and Jagadeesha RC

Abstract: In Karnataka, several landraces of banana are cultivated in different parts, known by different local names. Eight banana cultivars (Sugandhi Bale, Rajapuri Bale, Nanjanagud Rasbale, Sakkare bale, Basrai dwarf bale, Hanuman bale, Kalyani bale and Kari Bale) from Karnataka state were selected for genetic diversity study. Genetic variations and relationships among ten important banana cultivars were evaluated using 20 Inter Simple Sequence Repeat (ISSR) primers. The ISSR-PCR technique was used in the present study for the evaluation of genetic diversity within the Banana cultivars. Among 20 ISSR primers representing tri-, tetra, Penta and other repeats 12 primers revealed polymorphic patterns. Using those 12 primers, 72 bands in total were obtained, out of which 42 were polymorphic and scored giving an average of 3.5 bands amplified per primer. The used primers produced specific patterns for each banana cultivar. This study reveals there is a huge genetic dissimilarity among banana cultivars reveals their different genomic constitution and different evolutionary pattern of the cultivars. Hence need for cultivar wise cultivation, conservation, propagation and commercial production.

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