Author(s):

Nirja Thakur, Poonam Shirkot, Himanshu Pandey, Kanika Thakur, Vinay kumar and Rachappanavar

Abstract: First-generation sequencing was widely used during the 1980s and 1990s, but has been outdated in terms of output by NGS. These techniques are not time consuming and labour intensive as compare to first generation sequencing techniques.. In contrast to Sanger sequencing, the speed of sequencing and amounts of DNA sequence data generated with NGS, which is considered a “high-throughput technology”, are exponentially greater and are produced at significantly reduced costs. Third-generation sequencing uses parallel sequencing similar to NGS, but unlike NGS, third-generation sequencing uses single DNA molecules rather than amplified DNA as a template. Thus, third generation sequencing potentially eliminates errors in DNA sequence introduced in the laboratory during the DNA amplification process. The growing power and reducing cost sparked an enormous range of applications of Next generation sequencing (NGS) technology This review focuses on different techniques of next generation sequencing, their procedure on how they work as well as on their applications.

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