Author(s):

Chintankumar Chaudhari, Suman Kumar Jha, Ravindra Kumar Dhaka, Vipulkumar Parekh, MS Sankanur, Pravin Prajapat and Shikha Thakur

Abstract: In the present investigation, the molecular variability of teak in south Gujarat has been studied using ISSR marker. A total 12 primers produced clear and reproducible band out of 15 ISSR tested primers. The number of loci was ranged between 6 (UBC-888) to 14 (UBC-884) through amplification of these ISSR primers. Total 91 loci were polymorphic (80.53 %) and remaining 22 loci were monomorphic (19.47 %). Polymorphism percentage (PP) was ranged from 60 % for UBC-889 to 92.31 % for UBC-886. Maximum polymorphic information content value (0.36) EMI (5.68), MI (1.38) and resolving power (5.60) were observed with UBC-878, UBC-887, UBC-876 and UBC-886, respectively. Highest Jacard’s similarity value (0.68) was observed between Tapti and Vansada and Dharampur and Chikhali. However highest Nei & Li’s coefficient (0.84) observed between Bhenskatri and Kalibel. Two dendrograms of the genotypes constructed based on the Jacard’s and Nei & Li’s coefficient for clustering of the ten populations. All the genotypes could be divided in three major groups. The first group was further subdivided in to three subgroups: Ia) Tapti, Vansada and Bardipada-B population, Ib) Bardipada-A Ic) Chikhali and Dharampur, Bhenskatri and Kalibel. The second group (II) included Vyara and Kaprada populations. The results of PCoA corresponded well with the cluster analysis obtained through UPGMA. The first three coordinates accounted for 19.55, 16.07 and 13.12 per cent of the total variance, respectively. Thus, total cumulative variance was 48.75 per cent accounted by these three coordinates. Overall, the findings from the study is very useful for survival and conservation of teak genetic resources in threat of climatic change.

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