Author(s):

Amita Singh, Dr. Ajay Kumar and Dr. Vivek Srivastava

Abstract: Murraya Koenigii (Meethi Neem), Curcuma longa (Turmeric), Achyranthes aspera (Latjira), Withania Somnifera (Ashwagandha) and Syzygium cumini (Jamun) are being widely used in ayurvedic/traditional systems of medicines and source of several bioactive compounds. These plants are also used as culinary items. The present study was conducted to investigate the anti-bacterial effect of these plants against E. coli and P. aeruginosa are seem to cause many diseases in humans. We also considered the rhizospheric soil bacteria of these medicinal plants. In this project, we have done comparative studies of the metabolite extracted from five different medicinal plants on human pathogen i.e E. coli and P. aeruginosa. This was done by preparing the methanolic and water extract from dried leaves of these plants through soxhlet extractor and test them against the test micro-organisms through performing Disc assay. Two plants Murraya Koenigii (Meethi Neem) and Curcuma longa (Turmeric) showed good results and their both extracts i.e water and methanol gave no susceptible results against test micro-organisms The second finding was the effect of secondary metabolite extracted from the rhizospheric soil bacteria of same five plants on the test organism i.e P. aeruginosa and E. coli. During this total 20 colonies were isolated and out of 20 only 10 gave good result based on well diffusion test. Thus, this whole study revealed the antimicrobial effect of the metabolite and rhizospheric soil bacteria from these plants and so can be used to effectively to cure the disease caused by E. coli and P. aeruginosa.

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