Author(s):

B Jalandhar Ram, G Suresh Babu, ON Singh, G Roopa Lavanya and K Madhu Kumar

Abstract: Rice is the most important staple food crop for more than half of the population in the world. Crop improvement in rice depends on the magnitude of genetic variability and the extent to which the desirable genes are heritable. A total of thirty SSR markers were used to assess the extent of polymorphism and genetic diversity across the 34 rice genotypes. Out of the 30 SSRs, 25 markers were found to be polymorphic, whereas remaining was monomorphic. A total 86 alleles were detected from 25 SSR markers with an average of 4.12, which ranged from 2 (RM 10123, RM20354 and RM223) to 7 (RM232 and RM18571). The average PIC value was 0.50 at a range from 0.11 (RM 10123) to a maximum of 0.75 (RM232). Jaccard’s coefficient results showed that high amount of diversity existed between BPT2411*NDR359 and MTU1001 (81%), followed by the IR64 with BPT5204 and BPT2411*NDR359. The dendrogram based on the UPGMA analysis of NTSYS software grouped the studied rice genotypes into five major clusters. The grouping pattern showed that the clustering was done mostly according the parentage involved. Also there is a vide diversity existed between the parents and F1 progeny. Sufficient polymorphism revealed by the 25 SSR markers among the 34 rice genotypes in the present study justifies their use in genetic improvement programme, which depends on the extent of genetic variation. The genotypes which are found to be diverse based on both morphological and molecular diversity could be used for further breeding programme.

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