Author(s):

Carlo C Bondoc

Abstract: Most chemically-synthesized stains used in histological practices are expensive, hazardous to human and animal health, and threatening to the environment. Extraction of natural dye out of local plants have gained global interest and give promising tissue staining when outcomes are investigated. Curcuma longa rhizomes are proven to contain curcuminoids that exhibit various medicinal properties, and curcumin the bright yellow component of the plant that is being used as colorant. This natural dye from C. longa provides significant alternative as they are safer to use without health hazards, biodegradable, and have easy disposability. Nine biological specimens were used to compare the staining capacity of 15, 20, and 25-gram concentrations of C. longa extract and the commercial stain and were observed under microscope. The results revealed that the 15, 20, and 25-gram concentrations of C. longa stains do not have significant differences in the staining effect on various plant and animal tissues. Also, the staining effects of commercial and C. longa stains on monocot and dicot root and stem, leaf midrib, frog blood smear, and cycloid scale do not have significant differences Therefore, the alternative stain out of C. longa can be used as substitute to commercially available stains.

Pages: 3010-3017  |  4701 Views  3147 Downloads

Download (717KB)