Author(s):

A Matharasi, C Uma, P Sivagurunathan and P Sampathkumar

Abstract: In present study was aimed to utilize banana wastes residues (Banana peel, Banana pseudo stem and Spoiled banana) for the production of bioethanol by using potential indigenous ethanol genic yeast isolated Saccharomyces cerevisiae (KX033583) derived from spoiled banana. A totally 10 yeast strains (SB1, SB2 - SB10) isolated from spoiled banana. The 10 yeast strains with different colony morphology were selected and subjected to identification studies based on morphological and cultural characteristics. Among the 10 isolates screened for some attributes essential for ethanol production, the yeast strains SB10 recorded to possess all the attributes like fermentative ability, ethanol tolerance, and osmotolerance, flocculating ability, invertase activity and thermotolerance. The yeast isolates SB10 identified to species level by using rRNA sequencing studies and confirmed as Saccharomyces cerevisiae (KX033583). The SB10 employed in pretreated banana wastes materials for bioethanol production. Among the 3 substrates tested spoiled banana wastes showed better yield 23.42 ± 0.18gl^-1 this was followed by banana peel (17.00 ± 0.07gl^-1) and banana pseudo stem (15.61 ± 0.07g/l^-1). The parameter optima are important in any type of fermentation. Here an attempt was made to optimize different parameters like inoculums size, pH, and temperature and nitrogen source. The highest yield of ethanol production studied by inoculums level at 5%, pH is 6, temperature at 35^oC and Ammonium sulphate incorporate lead to rise in ethanol production.

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