Author(s):

Vijay Kumar Naik D, Bhaskara Reddy BV, Sailaja Rani J, Sarada Jayalakshmi Devi R and Hari Prasad KV

Abstract: The phytoplasma disease symptoms was observed on pigeon pea in experimental plots at Regional Agricultural Research Station, Tirupati, Andhra Pradesh, India. The causal agent of the little leaf disease was identified based on symptoms, amplification of 16S rDNA of the phytoplasma by nested PCR with primers P1/P7 and R16F2n/R16R2 and 1,800 bp and 1250 bp size products were amplified in first round PCR and nested-PCR respectively. The 1250 bp PCR product was cloned, sequenced and compared with the reference phytoplasma sequences collected from the database (NCBI). The 16S rDNA sequences of Andhra Pradesh isolate of little leaf of pigeon pea shared the highest nucleotide identity i.e., 98% with blackgram phyllody from Tirupati (KJ540943), which has been identified as 16SrDNA II group of phytoplasma. This is the first report of associate of ‘Candidatus Phytoplasma aurantifolia’of the 16Sr II group identifying pigeon pea from India.

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