Author(s):

S Sujatha, Dr. T Sekar and M Tamilarasan

Abstract: Toddalia asiatica belongs to the family of Rutaceae, stem extract of the plant, collected from Maruthamalai in Hill Western Ghats. Stem was investigated by established in vitro studies. Based the material and method quantitative determination of total phenolics, total flavonoids, and various in vitro antioxidant activities (DPPH, superoxide radical, reducing power, phosphomolybdenum and metal chelating ability) of methanolic extract was carried out using colorimetric methods. Results are evaluated as higher in stem of T. asiatica and recorded antioxidant as well as phytochemical quantitative of total phenol and flavonoid. The plant T. asiatica methanolic extract of stem showed greater antioxidant activity of IC₅₀ for DPPH assay (49.29 µg/ml) and compare to other extract, reducing power activity stem in methanol (0.647 EDTAE /100g extract), better in phosphomolybdenum reduction (57.4 mg/g acetone extract) and higher superoxide radical scavenging activity in methanolic extract (90.34%). However, the better metal chelating ability was shown by the methanol extracts of stem (4.30 EDTAE/100g) compared to other solvent extracts. The result indicates antioxidant and total phenol activity potential of T. asiatica, and concluded based of the GC-MS analysis of bioactive compounds.

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