Author(s):

Talib Saleh Al-Rubaye, Mohsen Hashim Risan, Dalal Al-Rubaye and Rabab O Radi

Abstract: The aim of this study is to characterize bioactive compounds from different Streptomyces spp. isolated from previous study from Tigris river sediment soil samples, the analysis done by HPLCs through using the crude extract compared to standards, which indicates the presence of four active compounds (Tetracycline, Clindamycin, Chloramphenicol and Rifampicin). While NMR data analysis performed by using the intracellular (biomass) extract due to more antagonism efficiency than that observed in extracellular crude extract, which revealed the presence of a clear peak for benzene ring at 7.24 ppm in sample N^-, D^- and M5. Most peaks between 1.56-1.58ppm in sample D related to the first Alkyle group CH3, while peaks between 1.68-1.89ppm are related to Alkane R3CH, RCH2R, RCH3 appeared in sample 21 and M5. Alkyle double bond or nitrogen or oxygen binding between peak 4.99-5.01ppm found in sample M5, D and 21. Sample N^- showed signals of Vinylic which represented in signals between 4.78 -5.17 ppm.

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