Author(s):

Jadhav PR, Soni KB, Sona Charles and Swapna Alex

Abstract: The present study was undertaken to enable the development of molecular strategies against BBrMV based on coat protein (CP) gene. The CP gene of Banana Bract Mosaic Virus (BBrMV) from infected Musa sp. var. Grand Naine (Kerala) was isolated and analysed using the BLAST tool. The fifty-six sequences showing 94-98% sequence similarity with this sequence were retrieved from Gen Bank database for divergence study. The phylogenetic analysis divided the isolates into two major clades with mean distance of 0.030. A conserved region of 234 nucleotides was identified in all the isolates. Neutrality test and secondary structure prediction of CP gene suggested purifying selection. The CP of all isolates showed the presence of conserved DAG motif while rad4 which functions in recombination and repair was found only in CP of isolate from Grand Naine (Kerala). CP structure was modelled using Modeller, I-tasser and swiss-modeller.

Pages: 1957-1962  |  895 Views  237 Downloads

Download (626KB)