Author(s):

Satish Sharma, Ajay Kumar, Pragati Saini, Reeti Singh and RK. Pandya

Abstract: Clusterbean is an important legume cultivated mostly on marginal and sub marginal lands of arid and semi-arid regions. Overall, India produces around 80% of global cluster bean production. The diseased samples were collected from Northern Madhya Pradesh for determining variability among collected twenty five isolates of Alternaria cucumerina var. cyamopsidis causal agent of Alternaria blight in Clusterbean based on conidial morphology and cultural variability on Potato dextrose agar medium. Out of twenty five isolates, five isolates viz: isolate A showed white with light olive black centre, isolate B was dark olive grey colour with white centre, isolate C and E were dark black and brown in colour respectively whereas the isolate D showed dark olive black colony with brown fluffy edge. On the basis of mycelial growth the isolate were found different in each other. The maximum conidial length was occurred in the isolate B (114.72 µm) and the conidial width was in the isolate B (7.0 µm). The maximum number of horizontal and vertical septa was associated with the isolate of D (7.16 µm and 4.48 µm respectively). The maximum radial growth of the colony was found by the isolate B (88.4 mm). Variability studies are important to document the changes occurring in populations and individuals as variability in morphological and physiological traits to indicate the existence of different pathotypes.

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