Author(s):

Manjula GS, Krishna HC, Karan M, Chirag Reddy M and Sadananda GK

Abstract: An investigation was carried out to know the biochemical changes in the extracted pigment from roselle (Hibiscus sabdariffa L.) calyces for edible colour during storage. For knowing the storage stability of extracted pigment, it was kept for 3 months in both ambient and refrigerated conditions. In storage under ambient and refrigerated condition, a decreasing trend were observed in the anthocyanin content, total phenols, water activity in the extracted pigment obtained by different methods of extraction upon storage under both conditions. At 90 days after storage at ambient and refrigerated condition, the anthocyanin content of 802.62 and 1091.92 mg 100 ml^-1 and total phenols of 4.29 and 5.56 mg ml^-1, were found be highest in treatment ethanol acidified with 1.5 N HCl. The water activity was found be highest 0.91 and 0.92 in the treatment of hot water extraction during 90 DAS under both conditions respectively. An increasing trend was observed in the total soluble solids of extracted pigment obtained by different methods of extraction upon storage and it was highest 29.30 and 28.93 °B was found in treatment ethanol acidified with 1.5 N HCl under both ambient and refrigerated conditions. Overall from the present investigation, the treatment of ethanol acidified with 1.5 N HCl was found to be the best with highest anthocyanin retention and total phenols compare to all other treatments which can be used for large scale extraction of biocolour from roselle calyces.

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