Are needed the present investigation were carried out on Commercial production with different ratio of sugarcane leaf with wheat straw on milky mushroom of two strain of Calocybe spp. (CI.-16-02, CI.-16-03). The result is present investigation shows that different substrates on yield of milky mushroom such as result indicated that in CI-16-03 maximum yield was observed in sugarcane leaf + wheat straw (2:1). Minimum days for spawn run (19.50days) were observed in sugarcane leaf + wheat straw (2:1). The Minimum days for pin head formation (21.75 days) were observed in sugarcane leaf + wheat straw (2:1). The Minimum days for first harvesting (27.00 days) were observed in sugarcane leaf + wheat straw (2:1). The Maximum number of pinhead initiation (62.25) were observed in sugarcane leaf + wheat straw (2:1). The Maximum numbers of fruiting bodies (24.75) were observed in sugarcane leaf + wheat straw (2:1). The Maximum average weight of fruiting bodies (27.42 gm) was observed in sugarcane leaf + wheat straw (2:1).