Material & Methods: A total 60 patients of Fistula in Ano were randomly selected, fulfilling the inclusion criteria. Total numbers of cases were randomly divided into three groups. Assessment was done on clinical improvement and improvement on growth of microorganism in the culture media.

Result: All the groups presented with similar improvement in decrease in amount of discharge at the end of therapy, however the degree of improvement was more marked in cases of Group A. Total leucocyte and neutrophil counts obtained at the end of therapy showed a highly significant and remarkable decrease in Group A and B.

Discussion & Conclusion: Decrease in the neutrophil and leucocyte count was comparable in Group A and Group B. E-coli was the most prominent organism responsible for infection in Fistula in ano. Group B revealed approx. 90% of the samples to be sterile at the end of the therapy while Group A revealed only 52% and only 10% were sterile in Group C, thus showing the marked efficacy of the antibiotic therapy as compared to that of Ayuvedic compound formulation used as a trial drug.