Author(s):

Madhusmita Pradhan, Shilpee Dhali, Priti Binita Lakra, Chinmay Pradhan and Santanu Mohanty

Abstract: Soil acidity contributes 70 per cent of the total cultivated land in Odisha. We have collected two hundred fifty (250) nos. of GPS based rhizospheric soil samples from Cuttack, Khordha, Balasore, Mayurbhanj, Keonjhar districts of Odisha and 106 nos. of samples showed pH below 5.5, which were screened for the enumeration and isolation of P- solubilizing bacteria. Five districts of Odisha were selected on the basis of their extent of soil acidity. Three districts viz; Cuttack, Khordha and Balasore with more than 80 % coverage of acid soils and the two with less than 50 % coverage were taken in to account for collection of rhizospher soil samples. The target soils of the five districts mostly come under the sub groups viz; Typic Ustorthents, Typic Tropaquepts, Vertic Tropaquepts, Typic Haplustalfs, Typic Ustochrepts, Typic Ustropepts, Vertic Ustochrepts, Typic Haplaquepts, Ultic Paleustalfs, Rhodic Paleustalfs, Aeric Ochraqualfs, Aeric Tropaquepts and Typic Rhodustalfs. A total of two hundred eight (208) nos. of PSB isolates were isolated from the villages of five districts. Out of 208, highest no. of PSB i.e. sixty five (65) isolates were isolated from Mayurbhanj district followed by fifty two (52) from Balasore district. Cuttack, Khordha and Keonjhar accounted for 36, 26 and 29 PSB isolates respectively. Forty five (45) PSBs with ≥ 180 % P solubilization efficiency on NBRIP agar medium were further screened for the P- solubilization efficiency on NBRIP Broth with Ca₃(PO₄)₂, FePO₄, Fe₃(PO₄)₂ and AlPO₄ as inorganic phosphate sources. Based on the soluble P recovery by the 45 PSB isolates at 48 and 72 h of incubation; five (5) isolates viz; BLS18 (Sarupala, Balasore), CTC12 (Echhapur, Cuttack), KHD08 (Balarampur, Khordha), KJR03 (Rangadihi, Keonjhar) and K1 (Chitrada, Mayurbhanj) from each district were selected for further screening and characterization of their P solubilizing efficiency. These native PSB isolates can be further characterized to screen their P solubilization efficiencies in field conditions.

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