Author(s):

Lovly MS and Merlee Teresa MV

Abstract: Nypa fruticans, Wurmb.is a mangrove palm and its plant parts are traditionally used by the local people curing herpes, toothe ache, head ache etc. Preliminary phytochemical screening of plant parts revealed the presence of variety of phytochemicals such as phenols, flavonoids, alkaloids, tannins, glycosides, terpenoids, coumarins and saponins. In the present study, hot extracts of leaves and stem of N. fruticans in different solvents such as water, methanol, ethyl acetate, chloroform and hexane were used to test the antimicrobial properties against B. subtilis, S. aureus, K. pneumoniae, E.coli, P. aeruginosa, A. flavus and A. niger. Antimicrobial study was conducted in different extracts by disc diffusion method. Different fractions of methanolic extracts in leaves were used to get the MIC value. Water extract obtained highest inhibition against both gram positive strains and gram negative strains. Maximum inhibition obtained in stem extract in water against B. subtilis (22.16±0.76mm). Lowest MIC of 5 µl or 0.125mg/100µl observed in S. aureus (18.00±1.21mm) and E. coli (15.00±1.29mm). Inhibition of leaf and stem extracts in different solvents against fungal strains were less compared to bacterial strains. High MIC recorded in both A. flavus and A. niger. Highest percentage of inhibition (56%) comparable with positive control (60%) obtained in 20 µl or 0.5 mg/100 µl concentration.

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