Author(s):

Ragavi K, Arulmozhi, Abalasubramaniam GA, Mekala P and Raja A

Abstract: Liver cancer is one of the most dreadful cancers with an incidence of one million cases every year in human beings. The currently available methods of treatment like surgery, chemotherapy and radiation induce significant side effects. So there is a need to explore an effective alternate therapies with minimal side effects. Hence, the present study was aimed to assess the in vitro anticancer effect of ethanolic extract of Annona muricata leaves which is being widely used in the traditional treatment of cancer in many countries. Phytochemical screening of ethanolic extract of Annona muricata leaves was carried out by using standard qualitative and quantitative methods. The in vitro anticancerous activity on HepG2 cell line was determined by MTT assay. Cells were treated with different concentration of the extract (0.01, 0.1, 0.3, 0.7, 0.9, 1, 3, 5 µg/ml) for 72 hours. Qualitative screening showed the presence of saponins, tannins, phenols, alkaloids, terpenoids, flavonoids, hydrolysable tannins and cardiac glycosides while amino acids, proteins, carbohydrates, phlobatannins, volatile oils, glycosides and vitamin C were absent. The quantitative screening revealed 3 mg of total alkaloid, 9 mg of phenol, 15 mg of tannin, 2.5 mg of flavonoid per gram of ethanolic extract. The IC₅₀ value of ethanolic extract of Annona muricata leaves was determined as1.116 µg/ml at 72 hours.

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