Author(s):

WGMM Jayawikrama, ARAMSSK Attanayake, YAAB Yapa, WD Ratnasooriya and RN Pathirana

Abstract: In Sri Lankan traditional medicine barks of Bridelia retusa (Family: Euphorbiaceae) is used to treat the rheumatoid arthritis and inflammation. However, this claim has not been scientifically validated or refuted yet. The aim of this study was to evaluate the antirheumatoid arthritic and anti-inflammatory activities of aqueous bark extract (ABE) of Bridelia retusa in vitro using heat induced protein (egg albumin) denaturation test which is widely used to access in vitro antirheumatoid arthritic activity and anti-inflammatory activity. ABE was made as described in Sri Lankan traditional medicine: 60g of dried bark was cut into small pieces and was boiled slowly in 1920 mL of distilled water approximately for 6 hrs until the volume was reduced to 240 mL. The solution was filtered using a muslin cloth. Different concentrations (31.25, 62.50, 125,250 and 500 µg/mL) of the ABE and reference drug diclofenac sodium (2500, 1250, 625, 312.50, 156.25, and 78.125µg/mL) were made and percentage inhibition of protein denaturation was accessed (N= 4). Further, phytochemical analysis was carried out using standard phytochemical techniques. The results revealed for the first time, that ABE of Bridelia retusa has marked and dose dependent (r2= 0.9653; P < 0.05) antirheumatoid arthritic and anti-inflammatory activities with an IC50 value of 197.8 µg/mL.

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