Author(s):

Gurinder Singh, Balbir Singh and Promila Mehta

Abstract: Jwarnashak Panch Kashya polyherbal formulation contained equal mixture of five selected plants such as Swertia chirata, Tinospora cordifolia, Zingiber officinale, Fumaria parviflora, and Cyperus rotundus has been used in the treatment of pyrexia. But the polyherbal formulation has not ever been evaluated scientifically to validate its traditional claims. Therefore, the present investigation was planned to evaluate antipyretic activity of polyherbal formulation in rats using brewer’s yeast-induced pyrexia model. Powdered polyherbal formulation was extracted successively and exhaustively using Soxhlet apparatus solvents to prepare n-hexane, chloroform, methanol and water extracts. Various extracts of polyherbal formulation were subjected to antipyretic activity evaluation in rats using brewer’s yeast-induced pyrexia model. The results of antipyretic activity showed that chloroform and methanol extracts possess significant antipyretic activity with respect to control and statistically similar to paracetamol because these extracts contain therapeutically active classes of phytoconstituents. n-hexane and water extracts did not show any sign of decline in temperature with respect to control. The ethyl acetate fraction was prepared from bioactive methanol extract to get flavonoids and phenolic compounds rich fraction. The ethyl acetate fraction and remaining bioactive extract were subjected to antipyretic activity. The ethyl acetate fraction possesses significant antipyretic activity at the dose of 162 mg/kg with respect to control, and statistically similar to paracetamol. The remaining bioactive extract did not exhibit antipyretic activity with respect to control. Finally, it can be concluded that bioactive chloroform extract and ethyl acetate fraction will be further subjected to column chromatography to extract bioactive fraction which contains antipyretic constituents act synergistically.

Pages: 3017-3020  |  997 Views  310 Downloads

Download (530KB)