Materials and Methods: Plant material was subjected to the extract preparation by soxhlet apparatus using ethanol. The various kinds of phytochemicals were studied and in vivo antidiabetic activity of Cordia gharaf bark were detected by using Dexamethasone induced insulin resistance in mice

Results: The study reveals the presence of phytochemicals such as carbohydrates, glycosides, flavonoids, alkaloids, steroids, tannins. Column chromatography using pet ether: acetone (90:10) and methanol: ethyl acetate (50:50) as mobile phase and all the collected fractions were subjected for TLC. The ethanol isolated compound of C.gharaf (300 mg/kg b.w) significantly reduces elevated blood glucose levels (P<0.0001). Treatment with the antidiabetic drug Glibenclamide (600 µg/ kg b.w). Blood samples were drawn from tail tip of mice at week interval till the end of study. Blood sugar level estimated by using one touch electronic glucometer. All the values of fasting blood sugar level and biochemical estimations were analyzed for one way ANOVA and post hoc Dunnett’s t test using graph pad prism 6.0 version computer software.

Conclusion: The present study establishes that the drug was found safe up to 2000mg/kg/po. 200mg/kg was found as lethal dose and 100,200, and 300 mg/kg was used for evaluation of anti-diabetic activity. 300 mg/kg dose was found to be effective for anti-diabetic activity. Cordia gharaf bark may contain the active constituent amongst the alkaloid, sterol, glycosides, and carbohydrate. It has significant anti-diabetic activity as compared to standard drug (Glibenclamide).