Author(s):

Pawan Kumar Panwar, J Bishnoi, VK Gaur, S Kumar, R Sharma, S Mehta and M Rizwan

Abstract: Genetic diversity and pathogenic variability among fourteen Fusarium udum isolates collected from different climatic conditions of India were studied. All the isolates exhibited variable levels of virulence against a susceptible pigeonpea cultivar (T-21). The genetic diversity allelic variations among these isolates were estimated using RAPD molecular markers. All the ten RAPD primers were found to be highly reproducible and produced a total of 114 bands of which 113 were polymorphic bands and showed 99.17% polymorphism. Primer OPG 3 amplified highest number of polymorphic bands (17). All the primers except OPG 4 showed 100% polymorphism. Genetic similarity was calculated using Jaccard's similarity coefficient and cluster analysis was used to generate a dendrogram showing relationships between them. The dendrogram grouped the isolates in two major groups A (12 isolates) and B (2 isolates) and the group (A) sub divided into three groups based on molecular analysis. The similarity coefficient values for fourteen isolates of F. udum ranged between 0.25 (DHO and BRC) to 0.61 (GHED and KNW). The average genetic similarity coefficient observed was 0.39±. The higher value of similarity coefficient close to one was indicative of close resemblance between the different isolates of F. udum. Result reflecting correlation of similarity with pathogenicity to some extent. However, there may not be a clear correlation of genetic molecular markers (RAPD) with the virulence, physiological characters and geographical region, however, studies indicate some correlation under specific instances of general nature as observed in the present study

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