Author(s):

Krupaliba Chudasama, Pritesh Bhatt, Kiran Chudasama and Vrinda Thaker

Abstract: The flowering plant family Euphorbiaceae has 300 genera and about 7,500 species, many of these plants are economically and medicinally important. A high range of medicinally important applications is reported for plant Euphorbia milii including a cure for cancer and wart. The varieties of this plant have distinct medicinal values. They can morphologically differentiate by its flower color but it is difficult to distinguish at vegetative stage. In the present study, Inter-Simple Sequence Repeat (ISSR) molecular marker study is performed to distinguish the varieties at vegetative stage. DNA was isolated from three varieties of Euphorbia milii using standard CTAB protocol. For molecular characterization, 30 different primers of ISSR were tested for all three varieties. The PCR amplification results obtained using ISSR primers for all three varieties showed unique band patterns on agarose gel electrophoresis. From the band patterns, monomorphic and polymorphic bands were statistical analysis. The clear and well-marked ISSR markers were coded in a binary form, 1 or 0 for the presences or absence of the bands, respectively and from these data, the varieties were distinguished. It is observed that ISSR technique is a useful molecular marker to distinguish Euphorbia milii varieties for best medicinal formulations and for clonal selection.

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