Author(s):

N Verma, Tajwar Izhar, M Chakraborty and JG Manjaya

Abstract: Two well recommended high yielding varieties of Soybean for Jharkhand namely, BSS-2 and RKS-18 were exposed to five different doses (50 Gy, 100 Gy, 150 Gy, 200Gy and 400 Gy) of gamma rays using cobalt 60 (Co⁶⁰) sources of Gamma chamber at Bhabha Atomic & Research Centre, Mumbai and their M₃ progenies were screened thoroughly. Morphological (viable) mutants were observed and recorded in M₃ generation from early seedling stage till physiological maturity with different doses of Gamma rays. Difference was observed between the varieties BSS-2 and RKS-18 in the degree of tolerance to the mutagens. The germination percentage was calculated in M₃ by counting the number of plants germinated after 15 days from the date of sowing in each progeny rows of the varieties, BSS-2 and RKS-18. Germination and survival percentage in both the varieties was lower as compared to control. Reduction in germination percentage was associated with increase in dose of mutagen in both varieties BSS-2 and RKS-18. It is evident that the survival percentage of plants decreased with the higher doses of gamma radiation. In variety BSS-2 at 100 Gy a higher survival percentage (94.20%) was recorded as compared to lower dose 50Gy (93.35%) while in RKS-18 the lower dose of 50 Gy(88.42) showed much higher survival percentage in comparison to higher doses of 150 and 400 Gy

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