Author(s):

Pallavi V and Abida PS

Abstract: The aim of the present study was to identify the differentially expressed, up regulated and down regulated genes by transcriptome analysis for drought tolerance. The genotypes PRS-64 (Angamali) was selected for molecular characterization among the ten genotypes through physiological characterization. The technique used to analyze the transcriptome was differentially display reverse transcriptase polymerase chain reaction (DD-RTPCR) which allows extensive analysis of gene expression among several cell populations (sturtevant 2000) ^[8]. The first stand cDNA was synthesized from the RNA samples using HT11G. Each first strand cDNA was used for the second amplification with eight different arbitrary primers. The PCR product was resolved in Urea polyacrylamide gels. The upregulated, down regulated and differentially expressed cDNA fragments were retrieved from the gel and reamplified with the same set of primers. The agarose gel electrophoresis showed that the (TDFs) transcript derived fragments obtained were relatively short (400-900 bp). TDFs were cloned using pGEMT vector and the clones were sequenced. The sequence from differentially expressed TDFs showed homology to copper containing amine oxidases which have a broader range of functions including cell signaling which contributes to terminal polyamines oxidation in peroxisomes. Polyamines are involved in growth development and response to abiotic stress.

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