Author(s):

R Shukla, EP Koshy and MD Ojha

Abstract: The rapid increase of population is not in tally with the increase of crop production which is leading the world to a severe food crisis. It has become the need-of-the-hour to increase the production of cereals by introducing traits like high yield, disease tolerance, submergence tolerance, etc. Mature de husked rice seed most suitably sterilized with HgCl₂ (0.1%) treatment for 5 minutes and observed that media containing 2, 4-D (1.5 mg/l) + BAP (0.5 mg/l) supported maximum callus induction and a percentage of 80% was obtained which was highly organogenic also. When the Somatic embryo of Swarna Sub1 inoculated in MS media supplemented with NAA (0.5 mg/l) +BAP (3 mg/l) + KIN (0.5 mg/l) produces average 10 shoot and 4 root initiation. The protocols developed will facilitate large scale propagation of the transformed plants.

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