Author(s):

Sweta Mishra

Abstract: An efficient and reproducible plant regeneration protocol was developed from axillary bud explants derived from physiologically mature trees of Jatropha curcas L., a promising tree for production of biodiesel. Direct organogenesis yields true to type plantlets. PVP (1.0 g/l), ascorbic acid (150 mg/l) and citric acid (50 mg/l) was used to effectively counter the phenolics released from the explants of the mature tree. The additives like adenine sulphate (25 mg/l) and glutamine (50 mg/l) helped to enhance the growth of Jatropha curcas explants. 91.65 ± 0.31 % shoot induction was observed in the axillary bud explants on MS medium supplemented with BA (2.00 mg/l) + Kinetin (1.00 mg/l) + GA₃ (0.10 mg/l). The maximum number of shoots (10.24±0.07) was observed in the MS + BA (2.00 mg/l) + Kinetin (1.00 mg/l) + GA₃ (0.05 mg/l). Maximum shoot length of 07.21 ± 0.25 was obtained on the MS medium supplemented with BA (2.00 mg/l) + Kinetin (0.50 mg/l) + GA₃ (0.10 mg/l). Profuse rooting of the regenerated shoots were obtained from the on the MS medium supplemented with IBA (1.00 mg/l) + NAA (1.00 mg/l) having a root induction response of 89.67±0.23 %. The highest number of roots (06.46±0.28) was obtained on MS + IBA (1.50 mg/l) + NAA (0.10 mg/l) having root length (04.53±0.25 cm). The survival percentage of the plants in the field after acclimatization was 80.0%.

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