Objective: The present work has been carried out to delineate the pharmacognostical profile of root, stem, and leaf of Lobelia alsinoides Lam.

Methods: Macroscopic, microscopic evaluation, powder analysis, fluorescence standards and quantitative evaluations of root, stem, and leaf of Lobelia alsinoides Lam. were carried out.

Results: Macroscopy and histological features revealed a dicotyledonous plant, having circular vascular bundles, idioblasts with prismatic crystals of calcium oxalate, air filled chambers in stem and anomocytic stomata in leaves were the diagnostic features of Lobelia alsinoides Lam. The presence of reticulate venation with small palisade ratio, starch grains and idioblasts are also the diagnostic characters of leaf. The distinguishing characters of triangular stem were the presence of idioblasts with adundant deposition of calcium oxalate crystals, simple starch grains, chlorenchyma and aerenchymatous cortex. Circular roots showed trabaculate cortex with prominent air cavities, annular xylem vessels with crystal fibres and simple starch grains.

Conclusions: The present study provides the scientific data on macro, microscopic, powder, quantitative and fluorescence standards for the proper identification and authentication of Lobelia alsinoides Lam.