Author(s):

Alen Godfrey R Jose, Abirami T, Kavitha V, Sellakilli R and Karthikeyan J

Abstract: Plant mediated synthesis of silver nanoparticles was important due to their medicinal applications. In the present study, silver nanoparticles were synthesized using a aqueous leaf extract of Asystasia gangetica and the production of AgNP’s was confirmed by the absorption spectrum of λ max at 430nm. The particles were characterized by scanning electron microscopy. The FTIR studies show the presence of various functional groups such as NH₂, OH, C=O groups, which are responsible for the reduction process. Disc diffusion method was followed to observe the bactericidal activity. The methanolic leaf extract of Asystasia gangetica shows moderate antibacterial activity against gram- negative bacteria, while the green synthesized silver nanoparticles shows a potential bactericidal activity against both gram-positive and gram-negative bacteria studied in the present investigation. The standard antibiotic amoxycillin was used as positive control and 1% DMSO were used as negative control. The bioactive compounds such as tannins and phenolic compounds present in the methanol extract and the nanoparticles capped with the bioactive compounds of plant material are responsible for the bactericidal activity. Further studies on characterization of specific compound responsible for the killing of bacteria, resulted in the invention of new compound to control the drug resistance organisms.

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