Author(s):

Krisnpal Singh Parihar, Chauhan MR and Barange PK

Abstract: Field experiment was conducted during the year 2016-17 at Post Graduate Institute Farm, Mahatma Phule Krishi Vidyapeeth, Rahuri (M.S.) to study the effect of consortium of endophytic nitrogen fixing bacteria on microbial population and other yield parameters of pre-seasonal sugarcane (second ratoon).The experiment was laid out in randomized block design with four replications and six treatments. There were four levels of nitrogen 0, 25, 50, and 100% of recommended dose of nutrients (RDF) with 100% P₂O₅, 100% K₂O along with and without foliar spray of consortium of endophytic nitrogen fixing bacteria. An Acetobacter diazotrophicus culture and consortium of endophytic nitrogen fixing bacteria (consortium includes Acetobacter spp., Azospirillum spp., Azoarcus spp., Agrobacterium spp., Burkholderia spp., Herbaspirillum spp.) for the fixation of nitrogen in plant biologically was obtained from Department of Microbiology, Vasantdada Sugar Institute, Manjari, Pune (M.S.) The application of 250 kg N, 115 kg P₂O₅ and 115 kg K₂O ha^-1 (RDF) to second ratoon sugarcane significantly increased cane and CCS yield as well as microbial population, followed by 25% N with foliar application of consortium of endophytic bacteria (T₄). The microbial population (Gluconacetobacter diazotrophicus) of sugarcane (top and cane) at 180 DAHR was ranged from 9.75 x10⁷cfu ml^-1 (T₁) to 34.25 x10⁷cfu ml^-1 (T₅).was ranged from 9.75 x10⁷cfu ml^-1 (T₁) to 34.25 x10⁷cfu ml^-1 (T₅). The microbial population depicts the role of N fixation and consortia in sugarcane by use of consortium by its population at 180 and at harvesting. Use of consortium results in 75% saving in N fertilizer even in second ratoon.

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