Author(s):

S Laxmi, MR Chambhare, S Kadlag, ML Ahire and TD Nikam

Abstract: Portulaca oleracea L. (Purslane), is a world’s most unscrupulous weed having very strong propagation method, stress tolerance and difficult to control by conventional methods. To ensure its control, we studied the weedy characteristics of P. oleracea by analyzing the re–emergence capacity after sunlight exposure for a month and influence of different concentrations of four herbicides viz. Oxyfluorfen (Goal), Glyphosate (Roundup), Pendimethalin (Stomp) and 2,4-dichlorophenoxyacetic acid (2, 4–D) on in vitro and naturally grown plants. The plants after removing from soil were place without watering and expose to sunlight (maximum intensity 550-600 watt /m²) for a month, showed the rapid re–emergence after plantation in soil and watering. The treatment of 2, 4–D and Oxyfluorfen were drastically reduce the betalain content. Pendimethalin did not have any significant effect on Chlorophyll content and betalain content. Initially, the activity of enzyme superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and guiacol peroxidase (GPX) in the herbicide treated plants was higher over the control. Later the sharp decline in enzyme activity was observed with the increase of contact time of herbicide to shoots of in vitro and plants grown in natural conditions. Both in vitro raised and plants grown in natural conditions were died with the treatment of 5% 2, 4–D and 5% Oxyfluorfen after 4 days of application. Our results provide evidence that P. oleracea has strong ability to survive in adverse conditions and 2, 4–D and Oxyfluorfen can be effective herbicide to its control.

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