Author(s):

Rajeev Ranjan, Shailesh Kumar and Ajay Kumar Singh

Abstract: Bacopa monnieri L. Penn. is an important medicinal crop. The problems with natural propagation is death of seedlings at two leaved stage, short viability of seed, marshy areas requirements and slow growth of stem cutting. Therefore, in the present investigation, an effort has been made to develop a rapid and reproducible protocol for the in vitro mass multiplication through callus induction of Bacopa monnieri. Nodal and leaf segments were used as explants for regeneration. A friable, compact, globular, morphogenic, green and white colored callus was initiated in different combination of media. For callus induction from leaf segments, MS basal media supplemented with 0.5 mg/l BAP and 0.5 mg/l NAA, supported the best result (75% callus induction) and MS salt supplemented with 4.0 mg/L BAP and 0.5 mg/L IAA supported best callus responses for nodal segment (85% callus formation). The MS salt supplemented with 1.0 mg/L BAP+ 0.5 mg/L NAA gave best results for shooting and multiple shooting induction from callus of leaf and nodal segment. Shoot induction percentage (85%), average number of shoots (15) and average shoot length (9.5 cm) was higher in case of callus developed from nodal segment as compared to leaf segments. Best performing rooting media (MS + 20g/l sucrose and 7g/l agar) induced rooting in 80% of shoots. Two weeks after hardening plants were transferred to soil in field/open conditions and 98% survival rate was observed, and the regenerated plantlets showed robust growth.

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