Author(s):

Debebe Worku Dadi, Shimelis Admassu Emire, Asfaw Debella Hagos and Frehiwot Teka Assamo

Abstract: M. stenopetala is a multipurpose plant having high nutritional and medicinal value. The aim of this study was to evaluate the effects of different drying methods and extraction solvents on the bioactive compounds in M. stenopetala leaves. Samples were dried using different drying methods and extracted using 50% and 70%ethanol, and 100%aqueous solvents. There was a significant difference (P< 0.05) among different drying methods and the extracting solvents. Freeze-dried samples gave the highest values of total phenolic contents (TPC) (57.2mgGAE/g dried powder) and total flavonoid contents (TFC) (130.30mgRE/g dried powder). The highest antioxidant activity was observed in freeze-dried 70% ethanol extract using DPPH and ABTS radical scavenging assays with the IC₅₀ values of 32.43µg/mL and 13.11µg/mL, respectively. It was followed by room-dried 70% ethanol extracts. Therefore, 70% ethanol was found to be the best extracting solvent to get higher yield of TPC and TFC with antioxidant effect.

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