Author(s):

Ram Lal Shrestha

Abstract: Fruit part of Piper longum Linn. was subjected to extraction of essential oil by hydro distillation in Clevenger apparatus. Methanol was used to extract the dried and powdered plant. The composition of essential oil so collected were determined by GC-MS system and showed the presence of 17 different compounds. The most abundant were germacrene (25.04%), heptadecene (11.71%), heptadecane (9.23%), β-bisabolene (8.36%) and caryophyllene (7.71%). Antibacterial activity of the essential oil of P. longum was studied. Oil exhibited moderate antibacterial activity against Klebsiella pneumoniae. The LD₅₀ of the sample was found to be 251.1μg/mL.

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