Arun Kumar Patel, Umakant Banjare, Ajjo Kumari, Sandeep Kumar Gupta, Khushboo Verma, Ashwani Kumar Upadhyay, Ramesh Kumar Singh and Kapil Deo Pandey

Leguminous crops producing pulses which are interacted whit Rhizobium and plant growth-promoting rhizobacteria (PGPR). This interaction promotes the growth and seed yield of pulse crops by fixing atmospheric nitrogen. Rhizobium forms nodules with leguminous crops to increase the nitrogen fixation capacity. Different presentive tests are oxidative, catalase, fermentative, methyl red and citrate for Rhizobium determination also BIOLOG GEN III microplate was used to presumptive determination of species of selected bacterial isolates by the carbon utilization pattern and these bacterial isolates showed production of different hydrolytic enzymes such as Amylase, Cellulase, Chitinase, Pectinase, and Lipase. These properties play important role in nodule formation which help in nitrogen fixation and the growth and development of the plant. Due to this property our isolated bacterial use as a biofertilizer.

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