Author(s):

Patil AG, Ambadkar CV, Kashid VS and Navgire KD

Abstract: The pathogenicity test of Xanthomonas axonopodis pv. punicae was performed with three different methods viz. Pin-prick method, Infiltration method and Spray inoculation method. Data revealed that amongst three different methods, infiltration method was found most suitable for development of symptoms which had taken 7 days for symptoms development. This method was followed by pin prick and spray inoculation method method which had taken 12 and 15.87 days, respectively for initiation of symptoms. Morphological characters of different Xap isolates were studied on nutrient agar media. Five isolates collected from different locations differed in respect of size of colony, shape of colony and colour of bacterial colony. Isolates Xap-1 and Xap-4 developed medium to large bacterial colonies. The isolates Xap-1 and Xap-3 developed small to medium sized bacterial colonies, whereas, Xap-5 produced small bacterial colonies. Xap-1 and Xap-3 produced small circular colonies, whereas, Xap-2 and Xap-4 produced circular to irregular bacterial colonies and Xap-5 produced circular bacterial colonies. Xap-1 and Xap-4 showed yellowish bacterial colonies, whereas, Xap-2 and Xap-5 showed light yellow coloured colonies and Xap-3 showed yellow coloured bacterial colonies.

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