Volume 2, Issue 1

 

Optimization of Microwave Assisted Extraction of Andrographolide from Andrographis paniculata and its Comparison with Refluxation Extraction Method

 

Author(s): Manvitha Mohan, Salma Khanam, B.G. Shivananda
1. Department of Pharmacognosy, East West College of Pharmacy, Bangalore 91, India.
2. Department of Pharmacognosy, Al-Ameen college of Pharmacy, Bangalore-27, India.

Abstract: A new method using Microwave assisted extraction technique has been developed for extraction of Andrographolide from Andrographis paniculata. Andrographis paniculata is a well-known plant of Ayurveda which is also known as king of bitters because of different bitter principles present in different parts of the plant and exhibits a wide spectrum of biological activities. The MAE conditions such as irradiation time, temperature and coarseness of powder were optimitized by means of orthogonal array design. The results suggested that the selected parameters were statistically significant. A comparative study was carried out to know the yield of andrographolide from the extracts prepared by microwave assisted extraction and refluxation using methanol and water as solvents. The amount of Andrographolide was estimated using HPTLC method. The results indicate that the extracts prepared by Microwave assisted extraction contained more amount of andrographolide when compared with refluxation extraction method.

 

Download Full Article : Click Here